run("Color Balance..."); setMinAndMax(0, 69);
run("8-bit");
        run("Enhance Contrast...", "saturated=0.4 normalize");
        run("Subtract Background...", "rolling=50");
        setAutoThreshold("Default dark");
        //run("Threshold...");
        setThreshold(50, 255);
        //setThreshold(50, 255);
        setOption("BlackBackground", true);
        run("Convert to Mask");
        run("Watershed");
	run("Analyze Particles...", " show=Outlines display");